Roronoa Zoro lost the use of his left eye during the two-year time skip period in the One Piece series while training under Dracule Mihawk, the world's greatest swordsman. The exact circumstances of how he lost his eye have not been revealed in the manga or anime. The first time the scar and his closed left eye appear is when he reunites with the Straw Hats after the time skip. Fans and sources speculate that the eye loss happened as part of his intense training, but Eiichiro Oda, the creator, has not disclosed the detailed story behind it. The closed eye is associated with mysterious theories, including potential hidden powers or Conqueror's Haki, but it remains one of the series' mysteries that might be revealed in future story developments.