According to the search results, there is no specific temperature that kills fruit flies. However, cold temperatures can stunt the development of new generations of fruit flies. When the temperatures fall below 53°F, these flies stop developing entirely. Very high temperatures above 100 degrees Fahrenheit, even for a few minutes, will kill adult fruit flies. While cold temperatures wont instantly kill household fruit flies, your average refrigerator is plenty cold enough to keep them from breeding. Below 60 degrees Fahrenheit, common fruit flies experience a sharp decrease in lifespan. Therefore, keeping fruits and veggies in the fridge and maintaining a dry, clean kitchen free of food refuse and sticky spills can help reduce fruit fly populations.