To sort the steps according to how a protein is processed and trafficked through the endomembrane system, here's the typical sequence:
- Protein synthesis begins on ribosomes attached to the rough endoplasmic reticulum (ER), where the protein is fed into the ER lumen during translation if it has an ER signal peptide.
- Inside the ER, the protein folds and undergoes initial modifications such as glycosylation.
- The protein is packaged into transport vesicles that bud off from the ER membrane.
- These vesicles travel to and fuse with the cis face of the Golgi apparatus.
- The protein is further modified and sorted as it travels through the Golgi stacks from the cis to the trans face.
- At the trans face of the Golgi, the protein is packaged into vesicles destined for different locations.
- Vesicles either:
- Fuse with the plasma membrane to secrete the protein outside the cell,
- Deliver the protein to lysosomes for intracellular digestion, or
- Transport membrane proteins to the plasma membrane.
This pathway reflects the secretory and endomembrane system trafficking route for proteins destined for secretion, the plasma membrane, or lysosomes.
If there is a provided list of specific steps to sort, please share it so I can arrange those exact steps according to this sequence.
